Induction of proopiomelanocortin mRNA expression in animal caps of Xenopus laevis embryos
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چکیده
منابع مشابه
Induction of cardiac muscle differentiation in isolated animal pole explants of Xenopus laevis embryos.
We have isolated a cDNA fragment encoding a portion of the myosin heavy chain alpha-isoform (XMHC alpha) in the amphibian, Xenopus laevis. The XMHC alpha transcript is highly enriched in adult heart RNA and is expressed exclusively in embryonic heart tissue. It therefore provides a tissue-specific marker for cardiac muscle differentiation during early embryogenesis. Using an RNAase protection a...
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Xenopus laevis is an ideal model system for investigating the mechanisms of pattern formation. The ability to express exogenous mRNA or introduce morpholinos into cleavage-stage Xenopus embryos has allowed gain- and loss-of-function experiments that reveal molecular-genetic control of development and regeneration. However, injection of mRNAs into cleavage-stage embryos provides limited spatio-t...
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In Xenopus, experiments performed with isolated ectoderm suggest that neural determination is a 'by default' mechanism, which occurs when bone morphogenetic proteins (BMPs) are antagonized by extracellular antagonists, BMP being responsible for the determination of epidermis. However, Ca(2+) imaging of intact Xenopus embryos reveals patterns of Ca(2+) transients which are generated via the acti...
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Embryos of Xenopus laevis reared in media with various low (less than or equal to 10(-5) M) magnesium ion concentrations will exhibit differing degrees of a potentially lethal magnesium starvation syndrome depending on the ion concentration and rearing temperature. The higher the rearing temperature or the lower the magnesium ion content of the medium the more severely the syndrome will be expr...
متن کاملExpression pattern of insulin receptor mRNA during Xenopus laevis embryogenesis
We have identified a member of the insulin receptor (InsR)/insulin-like growth factor-1 receptor (IGF-1R) family. The Xenopus insulin receptor (Xe-InsR) is present as a maternal 6.6 kb transcript. Northern blot analysis reveals the presence of this transcript until the mid-blastula transition (MBT), when levels decrease. At neurulation, two distinct transcripts of 6.6 and 7.7 kb are detected, b...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 2000
ISSN: 0012-1592,1440-169X
DOI: 10.1046/j.1440-169x.2000.00522.x